To determine this, we assessed the function of TMEM220-AS1 in HCC by performing a large sample validation in a population, followed by a series of cell function tests, dual luciferase reporter gene assay, bioinformatics analysis, western blotting, and RNA binding protein immunoprecipitation (RIP) to explore the underlying molecular mechanisms of TMEM220-AS1 function. Here, TMEM220 is linked to hepatocellular carcinoma.