The TLR4 and NF-κB proteins were qualitatively analyzed by immunohistochemical staining, and we found that staining for both TLR4 (Figures 6A, –C) and NF-κB (Figures 6D, –F) was stronger in the NEC group than in the control and NA groups. This evidence concerns the gene NFKB1 and necrotizing enterocolitis.