Based on the expression level of ARTN and that of the GFRα1, GFRα3, SDC3 and RET receptors in the screened CRC cell lines, we next generated stable clones of DLD1 and HCT116 cells with depletion of endogenous ARTN using siRNA-ARTN construct. Here, GFRA1 is linked to colorectal carcinoma.