Further, our subgroup analysis revealed that genotype frequency of the C allele of CD35 rs7525160 in HCC patients was significantly different from that in controls, especially in patients with AFP ≥ 400 ng/ml, tumor diameter of > 5 cm, TNM stage III/IV, cirrhosis, and portal vein thrombosis, which suggested that CD rs7525160 CC/CG genotype may be related to the type of HCC with poor prognosis. This evidence concerns the gene CR1 and hepatocellular carcinoma.