In this study, ECC-1 and Ishikawa cells have low expression of wild PTEN, but RL-95-2 cells, a PTEN mutant EC cell line, exhibited the similar sensitivity to metformin and NT1044 compared to ECC-1 and Ishikawa cells, suggesting that PTEN status may not play a big role in inhibition of cell growth in NT1044-treated endometrial cancer cells. This evidence concerns the gene PTEN and endometrial cancer.