In CHF rats, the NAD(P)H oxidase activity (Figure 4(a)) and NAD(P)H oxidase subunit NOX-2 (Figures 4(b) and 4(c)) and NOX-4 (Figures 4(b) and 4(d)) protein expression of CA, PA, and MA were increased significantly. This evidence concerns the gene CYBB and congestive heart failure.