Silencing salusin-β decreased the elevated NAD(P)H oxidase activity, NOX-2 and NOX-4 protein expression, and ROS levels in the arteries of CHF rats, which suggested that the activation of NAD(P)H oxidase and elevated ROS production played important roles in mediating the effects of salusin-β. The gene discussed is NOX4; the disease is congestive heart failure.