After the application of TNF-α to HPAECs, the eNOS activity (Figure 7(a)) and NO level (Figure 7(b)) in the cells were decreased, while the activity of NAD(P)H oxidase (Figure 7(c)) and the level of ROS (Figure 7(d)) were increased, which was consistent with the situation that occurred in CHF. The gene discussed is NOS3; the disease is congestive heart failure.