For example, Droplet Digital PCR has a higher sensitivity for ctDNA mutation detection than NGS, but can only be used to search for pre-determined specific mutations, such as in KRAS codons 12 and 13.36 We used a broad 57 gene cancer panel, including more amplicons than only the major PDAC hotspots and covering the entire TP53 coding region. The gene discussed is KRAS; the disease is cancer.