While the frequency of CD4+T cells in the two groups did not differ significantly, CD8+T cells in the CUMS group are significantly less than those in the control group (Table 3 and Figure 3), and a lower ratio of CD8+T cells/CD4+T cells was observed in the CUMS group, indicating that CUMS model can decrease the damage of cytotoxic T lymphocytes to bladder cancer cells. The gene discussed is CD4; the disease is urinary bladder cancer.