Besides, TMEM100 expression in normal colorectal cell line FHC and CRC cell lines HCT116, NCI-H498, SNU-C2B, and HCT-15 was detected, finding that compared with normal cell line, TMEM100 was markedly downregulated in CRC cell lines (Figure 1(d)). This evidence concerns the gene TMEM100 and colorectal carcinoma.