In the BRCA and NPC data sets, all three target genes had the same best reference gene combination: CIAO1, CNBP, HNRNPL, RER1, and SRSF4 in BRCA, and EIF1, MYL12B, RER1, RPS27, and SRSF4 in NPC. This evidence concerns the gene CIAO1 and nasopharyngeal carcinoma.