To verify our bioinformatics analysis results, we explored HOXB5 expression among four AML cell lines at both the RNA and protein levels and found that THP1 cells (with TP53 and NRAS mutations; without NPM1, FLT3, and DNMT3A mutations) had the highest expression level (Figure 4A). Here, FLT3 is linked to acute myeloid leukemia.