Furthermore, CCA cells may have different characteristic that can affect pathogenesis and outcome; for example, Li et al. (2016) identified down-regulation of FASN in intrahepatic cholangiocarcinoma while up-regulation of FASN was reported to enhance tumor aggressiveness in various cancer types including CCA, which is consistent with our study (Hao et al., 2014; Li et al., 2014; Yasumoto et al., 2016; Zaytseva et al., 2015; Zhang et al., 2020). Here, FASN is linked to intrahepatic cholangiocarcinoma.