STAT3 and in situ carcinoma: Western blot analysis of T24/DOX and T24/CIS tumor tissues revealed that the expressions of p-STAT3 (Y705), p-STAT3 (S727), P-gp, and Mcl-1 were reduced by NTX treatment as compared to vehicle group, but there was no significant difference in STAT3 (Figure 5F, 6F).