Western blot analysis of T24/DOX and T24/CIS tumor tissues revealed that the expressions of p-STAT3 (Y705), p-STAT3 (S727), P-gp, and Mcl-1 were reduced by NTX treatment as compared to vehicle group, but there was no significant difference in STAT3 (Figure 5F, 6F). This evidence concerns the gene MCL1 and in situ carcinoma.