The top 10 KEGG pathways enriched in obesity-NAFL-NASH sequence included cell cycle, Chemokine signaling pathway, Progesterone-mediated oocyte maturation, Oocyte meiosis, p53 signaling pathway, Viral protein interaction with cytokine and cytokine receptor, Toll − like receptor signaling pathway, Cellular senescence, Pertussis and Neuroactive ligand − receptor interaction (Fig. 3B). The gene discussed is TP53; the disease is non-alcoholic fatty liver.