Only eight pathways including p53 signaling pathway, AGE − RAGE signaling pathway in diabetic complications, PPAR signaling pathway, Endocrine resistance, Toll − like receptor signaling pathway, HIF-1 signaling pathway, Relaxin signaling pathway, and Apoptosis were significantly enriched in the health-NAFL-NASH sequence. This evidence concerns the gene PPARA and non-alcoholic fatty liver.