Furthermore, pre-injection of R7050 blocked the TNF-α-induced increase in the number of TUNEL-positive RGCs and partially reduced the number of apoptotic RGCs induced by IOP elevation, suggesting that TNF-α/TNFR1 signaling was indeed involved in RGC hyperexcitability and RGC injury in experimental glaucoma. This evidence concerns the gene TNFRSF1A and glaucoma.