The RT-PCR results showed that in patients 1, 3, and 5, HCAR3 was significantly increased in CRC tissues; in patient 2, there was no significant change between CRC tissue and normal tissue; whereas for patient 4, HCAR3 was significantly decreased in CRC tissues compared to the normal tissue (Fig. 3c). This evidence concerns the gene HCAR3 and colorectal carcinoma.