To further evaluate the mechanism by which FNDC4 regulates the PI3K/Akt pathway in HCC cells, Huh‐7 cells were transfected with full‐length FNDC4, extracellular truncated FNDC4 (Δ1‐188) and intracellular truncated FNDC4 (Δ168‐234) plasmids (Figure 5A). The gene discussed is FNDC4; the disease is hepatocellular carcinoma.