To further evaluate the mechanism by which FNDC4 regulates the PI3K/Akt pathway in HCC cells, Huh‐7 cells were transfected with full‐length FNDC4, extracellular truncated FNDC4 (Δ1‐188) and intracellular truncated FNDC4 (Δ168‐234) plasmids (Figure 5A). This evidence concerns the gene AKT1 and hepatocellular carcinoma.