There were no significant differences in the areas under the ROC curve between AFP and the two polypeptides for HCC from CHB (AFP vs. 1866 Da alone, P = 0.570; AFP vs. 4210 Da alone P = 0.191; AFP vs.1866 Da plus 4210 Da, P = 0.093).To evaluate the value of the two polypeptides in AFP-negative patients, the serum levels of 1866 Da and 4210 Da were analyzed in AFP-negative patients with HCC. The gene discussed is AFP; the disease is hepatocellular carcinoma.