ASCL1 and cancer: Using fragment information in the ASCL1 binding regions, we created a classifier that could be used to accurately detect 10 of 11 SCLCs (91% sensitivity, 95% CI = 65–99%) compared to 158 non-cancer individuals at >99% specificity (95% CI = 98–100%) (AUC = 0.92) (Fig. 5d).