Furthermore, qPCR assay (see Supplementary Table 1) of PWS genes in wild-type and PWS-IC mouse whole brain tissue at three developmental stages (E13.5, E18.5, P0), showed that Snord115, Snord116, Necdin had significantly reduced expression in the PWS-IC samples, while Ube3a was significantly overexpressed (Supplementary figure 2; t(6) = 11.788, p < 0.001; t(6) = −6.415, p < 0.001; t(6) = −24.033, p < 0.001; t(6) = −12.956, p < 0.001), as well as at stage P0 (t(8) = −3.573, p = 0.007; t(8) = −6.297, p < 0.001; t(8) = −7.4468, p < 0.001; t(8) = −6.415, p = 0.033). The gene discussed is SNORD116; the disease is Prader-Willi syndrome.