Specifically, we depleted Bcl9 in the MC38 and CT26 cell lines (supplementary Fig. 1a, b), as these express high β-catenin levels and are characterized by Wnt/β-cat dependent growth.35–37 Tumor growth in mice subcutaneously bearing CT26 or MC38 cells infected with Bcl9-shRNA was significantly suppressed compared to wild-type (WT) mice: tumor growth inhibition (TGI) rate of 73.8% by day 16 in the CT26 model and 83.9% by day 16 in the MC38 model. The gene discussed is BCL9; the disease is neoplasm.