As expected, with the increase of multiplicity of infection (MOI) from 1000 to 10,000, the HDR events steadily increased but no significant changes of total editing efficiencies at both EEF2 and AAVS1 loci (Additional file 1: Fig. S4c), accompanied by a considerable reduction of deletion indexes from ~ 2 to ~ 1% (Additional file 1: Fig. S4d). Here, PPP1R12C is linked to infection.