PTEN and hepatocellular carcinoma: In addition, compared with fabp10WT and pten KO fish, the heterozygous pten;tp53 cKO offspring (n = 262) showed obvious mortality at 1 month of age, and approximately 50% mortality by 2 months of age (Fig. 4c), suggesting that HCC formation, not developmental disruption, was the main cause of death in pten;tp53 cKO fish.