To achieve tissue-specific expression of pten and tp53 in zebrafish, we established an HCC model in a Cre-loxp transgenic zebrafish line via a CRISPR/Cas9 system directly targeting tumour suppressor genes in zebrafish liver tissues through Cas9-mediated mutation of the pten and/or tp53 locus under the control of the fabp10 promoter. The gene discussed is PTEN; the disease is neoplasm.