We further uncovered that the miR-624/ARRDC3/YAP axis increased HIF1α expression to enhance ESCC chemotherapy resistance as judged by an elevation in HIF1α expression and promoted cell proliferation, invasion, versus a reduction in apoptosis after ESCC cells were treated with upregulated miR-624 or YAP and PT. This evidence concerns the gene F2 and esophageal squamous cell carcinoma.