In our study, knocking down endogenous GINS1 with shGINS1 increased the sensitivity of HCC cells to Sorafenib, and restoring HRAS or stem associated pathway partly recovered the sorafenib resistance, suggesting the sorafenib resistance of HCC cells at least partially mediated by GINS1-induced CSCs. This evidence concerns the gene GINS1 and hepatocellular carcinoma.