LDHA and infection: After 24 hr of infection, cells were treated with 2.5 μM of eugenol oleate in combination with 0.3125 μM of amphotericin B in vitro. The levels of GLUT-1, HK-1, HK-2, HK-3, PFKM, PFKB-3, and LDHA were studied by using the semi-quantitative RT-PCR method.