To this end, an integral analysis of the mitochondrial SCCAs oxidation was carried out in two widely used human CRC lines, COLO 205 and HCT 116 cells, by assessing the activity of acetate thiokinase (AcK), the initial step in the acetate oxidation pathway, the level of several OxPhos enzymes and their acetylation profile, and the OxPhos flux. Here, TNK2 is linked to colorectal carcinoma.