In order to study the mechanism of GAS5 in degenerative NPCs, we first predicted with the LncATLAS software that GAS5 might mainly be located in the cytoplasm (Figure 3A), while the FISH experiment and nuclear-cytoplasmic RNA separation further clarified that GAS5 was mainly in the cytoplasm of NPCs (Figures 3B,C), suggesting that lncRNA GAS5 may act as a ceRNA in IDD. The gene discussed is GAS5; the disease is intervertebral disk degenerative disorder.