In this study, we fabricated NF‐κB‐p65‐targeted MBs by decorating NF‐κB‐p65 antibody molecules onto the surface of MBs, and NF‐κB‐targeted CEUS was performed on murine models with chemically induced colitis to quantify NF‐κB‐p65 expressions of colon tissues, aiming to explore its potentials in the evaluation and treatment monitoring of IBD. The gene discussed is RELA; the disease is inflammatory bowel disease.