Therefore, the computed multiple model formula (Formula 1) could be used to predict ALS patient Treg count after three 2MIU-IL-2 administration cycles once the baseline level of CD27 and TLR9 expression is known.Formula 1: Treg number D64=724.57+59.49 * CD27+(-625.00 * TLR9). The gene discussed is TLR9; the disease is amyotrophic lateral sclerosis.