To study the dynamic change of active enhancers during HNSCC transformation, we utilized a common-used HNSCC mouse model, a transgenic mouse with a combined Tgfbr1/Pten knockout (K14-CreERtam+/−; Tgfbr1flox/flox; Ptenflox/flox). Here, KRT14 is linked to head and neck squamous cell carcinoma.