To deeply investigate the biofunction of CLDN6, we conducted the GO and KEGG pathway enrichment analyses of its 50 targeted binding proteins, revealing that the BP was major in cell–cell adhesion or bicellular tight junction, its MF was primarily involved in virus receptor activity, hijacked MF, miRNA binding, and regulatory RNA binding, and the main pathways were enriched in leukocyte transendothelial migration, tight junction, cell adhesion molecules, hepatitis C, and pathogenic E. coli infection. This evidence concerns the gene CLDN6 and escherichia coli infection.