Finally, an eight-gene (N4BP3, NRGN, ITGB5, FAM110D, LPCAT1, CASQ2, UNC5B, and SLCO2A1) prognostic signature was constructed under the optimal value of λ (Supplementary Figure 4C) to obtain the risk score for HCC samples. This evidence concerns the gene N4BP3 and hepatocellular carcinoma.