Although the expression level of interferon-γ signaling increased in the U373 MG cell line in the NGS data analysis, the expression level of PDL1 gene did not increase over time, suggesting that without T cell infiltration, the 6 Gy single dose irradiation to the U373 MG glioblastoma cell line did not upregulate PD-L1 (Table 1). This evidence concerns the gene CD274 and glioblastoma.