STAT3 and hepatocellular carcinoma: To verify whether the involvement of JAK-STAT3 signaling pathway, an important pathway associated with cancer metastasis, in the oncogenic role of CKS1B in HCC progression, the western blot analysis was used to detect the effect of abnormal expression of CKS1B on the expression of JAK-STAT3 signal pathway in Hep3B and Huh7 cells (Figure 4 and supplemental Figure S1).