To overcome the limitation of these technical approaches, we have recently used the Sglt2-Cre/Agtr1a-foxed recombination to delete AT1 (AT1a) receptors selectively in the proximal tubules of the kidney and to determine the specific roles of intratubular Ang II and AT1 (AT1a) receptors in basal blood pressure homeostasis and the development of hypertension induced by circulating or intracellular Ang II (Rubera et al., 2004; Li et al., 2011b, 2021; Rateri et al., 2011). This evidence concerns the gene SLC5A2 and hypertensive disorder.