For instance, 20-HETE leads to eNOS uncoupling and impairs acetylcholine-induced relaxation through tyrosine kinase-, MAPK/ERK-, and IKK- dependent mechanisms that involve HSP90-eNOS dissociation and HSP90-IKKβ association (Cheng et al., 2010) leading to endothelial dysfunction. Here, NOS3 is linked to endothelial dysfunction.