Next, we used TUNEL staining and WB analysis to investigate the effects of SKI and ROS on apoptosis in the renal tubular cells as indicated by staining in the renal tubular epithelial cells and changes in the protein expression levels of Bax, Bcl-2, and caspase12 in the kidneys of the rat model of DKD. This evidence concerns the gene BAX and diabetic kidney disease.