Flow cytometry analysis showed that S18 displayed high levels of mesenchymal markers CD90, CD146, CD105 and CD166, while S26 showed relatively low levels of these mesenchymal markers, confirming that S18 comprises a high proportion of cancer stem-like cells with mesenchymal features, while S26 cells are mainly in an epithelial state (Fig 2A). This evidence concerns the gene ENG and cancer.