In chow‐fed mice, there was no significant change in UCP1 and PRDM16 in all the three fat depots either with END or HIIT (Figure 4a–c), which contrasts with a study reported by Stanford et al where there was an increase in UCP1 mRNA (Stanford et al., 2015) and PRDM16 protein (Vidal & Stanford, 2020) in animals with established obesity before exercise. The gene discussed is PRDM16; the disease is obesity due to melanocortin 4 receptor deficiency.