Piperlongumine reverses the R273H mutant P53 protein by overproducing ROS and leads to a restoration of the functional status of mutant P53 in human colon cancer cells, inducing the transcription of the P53 target gene, murine double minute 2, and BAX and finally resulting in cancer cell apoptosis 125. Here, TP53 is linked to malignant colon neoplasm.