ANGPTL4 and colorectal carcinoma: RT-qPCR and Western blot results displayed that shANGPTL4 treatment could reduce the expression of ANGPTL4 in the two CRC cell lines, and the silencing efficiency of shANGPTL4-1 was highest, so the shANGPTL4-1 sequence was selected for subsequent experiments (Figure 3A, B).