Western blot and RT-qPCR results revealed that the expression of epithelial markers E-Cadherin and ZO-1 was significantly down-regulated, and the expression of mesenchymal cell markers vimentin and N-Cadherin was significantly up-regulated in CRC cells after shNAGPTL4 treatment (Figure 3F, G). The gene discussed is VIM; the disease is colorectal carcinoma.