To investigate the effect of Sirt3 on the radiation sensitivity of lung cancer cells, we constructed Sirt3 knockdown shRNAs and overexpression plasmid, and further transfected it to A549 and H1299 cells by lentivirus infection, then verified their knockdown and overexpression efficiency by Western Blot (Fig. 2A). The gene discussed is SIRT3; the disease is lung cancer.