In addition, detection of parasites DNA or RNA using polymerase chain reaction (PCR) method utilizing primers derived from parasites mitochondrial cytochrome b (cytb) gene could be more common reliable and widely used in diagnosing the infections particular in laboratory for high sensitivity and specificity even when blood smears are negative in case of low parasitemia or early stage of infection in animals9,12,20–22. The gene discussed is MT-CYB; the disease is infection.