In control BMDMs and RAW264.7 cells infected with the WT actA:GFP reporter strain, the majority of bacteria were localized to the cytosol at 4 h post-infection (p.i.), and displayed robust transcription of actA, visualized by the expression of actA:GFP and localization of ActA protein on the bacterial cell surface, as expected (Fig. 3b, Supplementary Fig. 3d, e). This evidence concerns the gene ACTA1 and infection.