Besides, to confirm that CCL21 mRNA is confined inside the urinary small EVs, EVs pellet was treated with RNase in the absence or presence of Triton X. The results showed that CCL21 mRNA expression in urinary small EVs from DN patients was not diminished after RNase treatment, but was sharply decreased after membrane disruption when both Triton and RNase were applied (Fig. 1F). This evidence concerns the gene CCL21 and liver dysplastic nodule.