NRXN1 and intrahepatic cholangiocarcinoma: However, since neurexin-1β was detected in 4/11 cultures (mean 2-ΔΔCt = 0.06194, range 0.00224–0.72034) we decided to use a pan-neurexin-1 antibody which detects both neurexin-1α and neurexin-1β for Western blotting, IHC and ICC.