To further define the function of IFI30, we constructed breast cancer cell lines with stable knockdown of IFI30 using lentiviral infection assay, CCK8 and clone formation assay confirmed that knockdown IFI30 could inhibit the proliferation of breast cancer cells, and transwell assay confirmed that knockdown IFI30 could inhibit the migration and invasion of breast cancer cells. The gene discussed is IFI30; the disease is breast cancer.