FOSL1 and colorectal cancer: To this end, we first identified high-confidence FOSL1/FRA-1 binding sites in chromatin immunoprecipitation-seq (ChIP-seq) previously generated in the KRAS mutant HCT116 colorectal cancer cell line (see Materials and methods), and then we determined the counts per million reads (CPM) of the enhancer histone mark H3K27Ac in a set of MES (n = 10) and non-MES BTSCs (n = 10) (Mack et al., 2019), selected based on the highest and lowest FOSL1 expression, respectively.