Then, we transfected LncRNA MEG3 and miR-543 into NSCLC cells to detect their biological behavior and found that the proliferation and invasion ability, IDO1, IDO2, TDO, and autophagy-related Beclin-1 and LC3-II proteins of NSCLC cells overexpressing LncRNA MEG3 decreased evidently, while the autophagy-related LC3-I protein increased evidently, suggesting that LncRNA MEG3 acts as a tumor suppressor gene in NSCLC. The gene discussed is MEG3; the disease is non-small cell lung carcinoma.